Lifeng Peng

Proteomics School of Biological Sciences

Courses

Teaching in 2017

Dr Lifeng Peng profile picture

Research interests

My research interest is mass spectrometry based proteomics and metabolomics and their applications in analyzing mammalian and microbial systems to improve medicine and biotechnology and to study their physiology. This includes:

  • Proteomics. My lab has well-established expertise in mammalian, plant and microbial proteomics platform for large-scale characterization and quantification of cellular proteomes responsible for different physiological stages, or normal and diseased/treated conditions.
  • Metabolomics. My lab is developing metabolomics methods for quantitative analyses of primary and secondary metabolites using LC mass spectrometry to study the metabolic profiles and changes of cells in response to stimuli.
  • Metabolic pathway analysis. This involves in applying 13C tracers and other stable isotopes to cell cultures and analyzing the mass isotopomer distributions of the intracellular metabolites using GC-MS and LC MS/MS and eventually quantifying the metabolic fluxes in the complex pathways. The mass isotopomer distributions offer direct information about the metabolic activities of specific pathways and the metabolic fluxes give the global quantification of the entire metabolic network. These techniques offer sensitive and quantitative analysis of cellular metabolism.

My current research project includes the study of non-alcoholic fatty liver disease (NAFLD). We explore the mechanisms of lipid accumulation in liver cells and the molecular responses to lipid-filled liver cells to provide information leading to the development of therapeutic drugs to treat the disease. Experimental approaches include HepG2 cell cultures, microscopy for detection of cellular change and mass spectrometry for analyses of proteins, metabolites, isotopermer distributions and metabolic fluxes.

Selected publications

1. He L, Xiao Y, Gebreselassie N, Zhang F, Antoniewicz MR, Tang YJ, Peng L. Central metabolic responses to the overproduction of fatty acids in Escherichia coli based on 13C-metabolic flux analysis. Biotechnology and Bioengineering. 2014, 111: 575–585.

2. Ahmed S, Zhang M, Peng L. Improving feature ranking for biomarker discovery in protoemics mass spectrometry data using genetic programming. Connection Science. 2014.

3. Smit A, StrabalaTJ, PengL, RawsonP, Lloyd-JonesG,Jordan TW.Proteomic phenotyping of novosphingobium nitrogenifigens reveals a robust capacity for simultaneous nitrogen fixation, polyhydroxyalkanoate production, and resistance to reactive oxygen species. Applied and Environmental Microbiology, 2012, 78:4802-4815.

4. Rawson P, Stockum C, Peng L, Manivannan B, Lehnert K, Ward HE, Berry SD, Davis SR, Snell RG, McLauchlan D, Jordan TW. Metabolic proteomics of the liver and mammary gland during lactation. J Proteomics, 2012, 75:4429-4435.

5. Peng L, Kapp EA, McLauchlan D, Jordan TW. Characterization of the Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative Standard using SDS-PAGE shotgun proteomics. Proteomics, 2011, 11:4376-4384.

6. Wilmes A, Rawson P, PengL, McLauchlanD, Northcote PT, Jordan TW and Miller JH. Effects of the microtubule stabilizing agent peloruside A on the proteome of HL-60 cells. Investigational New Drugs, 2011, 29:544-553.

7. Mast S, Peng L, Jordan TW, Flint H, Phillips L, Donaldson L, Strabala TJ, Wagner A. Proteomic analysis of membrane preparations from developing Pinus radiata compression wood. Tree Physiology. 2010, 30:1456-68.

8. Peng L, Rawson P, McLauchlan D, Lehnert K, Snell R and Jordan TW. Proteomic analysis of microsomes from lactating bovine mammary glands. Journal of Proteome Research, 2008, 7:1427-1432.

9. Beddek AJ, Rawson P, Peng L, Snell R, Lehnert K, Ward HE and Jordan TW. Profiling the metabolic proteome of bovine mammary tissue. Proteomics, 2008, 8:1502-1515.

10. Peng L, Arauzo-Bravo MJ and Shimizu K. Metabolic flux analysis for a ppc mutant Escherichia coli based on 13C-labelling experiments together with enzyme activity assays and intracellular metabolite measurements. FEMS Microbiology Letters, 2004, 235:17-23.